image manipulation – Retraction Watch

Fabricated allegations of image manipulation baffle expert

*The fabricated claim about image manipulation raises a question: Why bother?*

Mike Rossner had never seen anything like it. At first, the anonymous comment on PubPeer, which claimed a lane of a western blot in a research paper had been duplicated, seemed nothing out of the ordinary to Rossner, who specializes in detecting image manipulation in biomedical research. The surprise came when he looked closer at the magnified images the commenter had provided to support their allegation.

While the two enlarged lanes in the anonymous comment were indeed identical to each other, close inspection of the original image from the paper, which the comment included, clearly showed two different lanes. It wasn’t hard to see how the fakery had been achieved: A single lane had been copied and pasted on top of an adjacent lane.

“I have looked at thousands of PubPeer allegations, and this is the first time I have come across what appear to be fabricated allegations,” Rossner told us.

Dana-Farber settles suit alleging image manipulation for $15 million

Dana-Farber Cancer Institute has settled a lawsuit filed under the False Claims Act, admitting researchers used images and data that were “misrepresented and/or duplicated” in support of grant applications to the National Institutes of Health. Dana-Farber agreed to pay $15 million to settle the claim.

Sleuth Sholto David filed the claim in April 2024, about three months after he first posted about the allegations, which played a key role in Dana-Farber’s decision to retract or correct dozens of studies. Authors of some of those papers were among senior leaders of the institution, including president and CEO Laurie Glimcher.

As is typical in such cases, the complaint remained sealed while the Department of Justice investigated. As part of the agreement, David will receive $2.63 million, or 17.5 percent of the settlement.

Widespread image reuse, manipulation uncovered in animal studies of brain injury

One of the papers in the analysis contained a figure (bottom) found to have overlap with other work by the same author (top). Both papers have been retracted.
*Annotated images: PubPeer*

More than 200 papers on ways to prevent brain injury after a stroke contain problematic images, according to an analysis published today in PLOS Biology. Researchers found dozens of duplicated Western blots and reused images of tissues and cells purportedly showing different experimental conditions — both within a single paper and across separate publications.

As we reported last year, René Aquarius and Kim Wever, of the Radboud University Medical Center in the Netherlands, first noticed these patterns in 2023 when they started working on a systematic review of animal studies in the field. They had wanted to identify promising interventions for preventing early brain injury following hemorrhagic stroke. Instead, their efforts turned into an audit of suspicious papers in their field.

Of the 608 studies they analyzed, more than 240, or 40 percent, contained problematic images. So far, 19 of those articles have been retracted and 55 corrected, mostly from the researchers’ efforts to alert journals and publishers about the issues. Almost 90 percent of the problematic papers had a corresponding author based in China, and many appeared in major journals such as Stroke, Brain Research and Molecular Neurobiology.

Exclusive: Journal to retract Alzheimer’s study after investigation finds misconduct

A journal says it will retract a 2019 paper on an Alzheimer’s treatment after an institutional investigation found research misconduct, according to emails seen by Retraction Watch. The move comes four years after another investigation by the same university uncovered image duplication in a different paper by a similar group of authors.

The paper, published in Biological Psychiatry, describes the potential of an apoE antagonist for treatment in Alzheimer’s disease.

A 2019 news release by the University of South Florida, home to several of the researchers involved in the study, called the work “promising.” Lead author Darrell Sawmiller, an assistant professor at USF, said the study represented “the first time … we have direct evidence” apoE “acts as an essential molecule” in the mechanisms leading to Alzheimer’s.

Second study using ‘Tin Man Syndrome’ X-ray under scrutiny following Retraction Watch inquiry

An altered image posted as an April Fool’s joke (left) was used as a figure (right) in a 2021 paper in *Scientific Programming*.

Just as The Wonderful Wizard of Oz was the first of 14 books in a series, our recent coverage of a paper on “Tin Man syndrome” seems to have sequels. After we wrote about a case study describing a man with his heart in his abdomen retracted for plagiarizing images from an April Fools’ joke, a reader flagged yet another paper using the same image.

As we previously reported, the authors of a “rare case report” appearing in Medicine claimed they had encountered a case of a man with asymptomatic “ectopia cordis interna,” in which his heart was in his abdomen. After the article was retracted, the corresponding author admitted the photos had been taken from a 2015 April Fools’ paper in Radiopaedia describing the same (fictitious) condition.

Following that coverage, a reader did a reverse image search of the X-ray in both papers and found a 2021 article from Scientific Programming, published by Wiley. The study recommends a non-conventional ventilation option for treating neonatal respiratory distress syndrome. The paper has been cited twice, according to Clarivate’s Web of Science.

Deputy department chair loses paper for image duplication, more retractions to follow

An Elsevier journal has retracted a paper coauthored by a deputy department chair at Thomas Jefferson University in Philadelphia and says it plans to retract at least two more of his articles for image-related concerns.

The 2022 paper, in Matrix Biology, describes the regulatory role of proteoglycans in remodeling of the cervix during pregnancy. According to the August 12 retraction notice, 18 of the image panels were duplicates. The paper has been cited 18 times, according to Clarivate’s Web of Science.

Renato Iozzo, deputy chair of Pathology, Anatomy & Cell Biology at Jefferson, is a coauthor on the study. Neither Iozzo nor corresponding author Mala Mahendroo, a researcher at UT Southwestern Medical School in Dallas, responded to our requests for comment.

Director of Cambridge toxicology institute retracts paper for potential image manipulation

Twelve years after sleuths flagged problematic images in a 2009 paper, the authors — including the head of a UK research institute — have retracted the article.

The paper, published in Genes & Development, has been cited 126 times, according to Clarivate’s Web of Science.

According to the June 1 retraction notice, the authors retracted the paper because of “anomalies in the data presented” in multiple figures. “The issues relate to potential instances of image manipulation, including undisclosed splicing, lane flipping, and lane and panel duplications in the preparation of these figures.”

COPE integrity officer loses 22-year-old paper for image concerns

The concerning figure from the paper, Fig. 2A, with increased contrast, courtesy of “Mycosphaerella arachidis” on PubPeer.

A journal has retracted a 22-year-old-paper whose first author is the integrity officer for the Committee on Publication Ethics over concerns about image editing that “would not be acceptable by modern standards of figure presentation.”

The 2003 paper, “A recombinant H1 histone based system for efficient delivery of nucleic acids,” was published in Elsevier’s Journal of Biotechnology and has been cited 41 times, according to Clarivate’s Web of Science.

Sleuth Sholto David, who goes by the name “Mycosphaerella arachidis” on PubPeer, raised concerns about the image in December 2023, pointing out a “[d]ark rectangle” that appeared to be “superimposed onto the image.”

Fourth retraction for Italian scientist comes 11 years after sleuths flagged paper

PLOS One has retracted a 2011 paper first flagged for image issues 11 years ago. The retraction marks the fourth for the paper’s lead author, Gabriella Marfè of the University of Campania “Luigi Vanvitelli,” in Caserta, Italy.

“Involvement of FOXO Transcription Factors, TRAIL-FasL/Fas, and Sirtuin Proteins Family in Canine Coronavirus Type II-Induced Apoptosis,” has been cited 41 times, according to Clarivate’s Web of Science.

Elisabeth Bik flagged the article on PubPeer in 2014 for apparent image manipulation and duplication in six figures. In a 2019 email to PLOS staff, pseudonymous sleuth Claire Francis drew attention to Bik’s findings. The journal retracted the paper on May 6 of this year.

‘Anyone can do this’: Sleuths publish a toolkit for post-publication review

For years, sleuths – whose names our readers are likely familiar with – have been diligently flagging issues with the scientific literature. More than a dozen of these specialists have teamed up to create a set of guides to teach others their trade.

The Collection of Open Science Integrity Guides (COSIG) aims to make “post-publication peer review” more accessible, according to the preprint made available online today. The 25 guides so far range from general – “PubPeer commenting best practices” – to field-specific – like spotting issues with X-ray diffraction patterns.

Although 15 sleuths are named as contributors on the project, those we talked to emphasized the project should be largely credited to Reese Richardson, the author of the preprint.

Share this:

Share this:

Share this:

Share this:

Share this:

Share this:

Share this:

Share this:

Share this:

Share this: